EPA Method A in the EPA methods list database. View all EPA methods. EPA Method A: Chlorinated Biphenyl. Congeners by HRGC/HRMS. Horizon Technology, Inc., Salem, NH. Horizon Technology, Inc., 45 Northwestern Dr. Ecology may require or allow the use of the most current accepted revision of EPA Method (USEPA, ) at contaminated sites, instead.
If a sufficient number of volunteer laboratories are not identified, EPA may consider issuing contracts with one or more qualified laboratories through a competitive bidding process.
Because very low levels of chlorinated biphenyls CBs are measured by the method, the elimination of interferences is essential. Interfering compounds may be present at concentrations several orders of magnitude higher than the CBs. The RSDs generally are higher than those in Method A for some of the low molecular weight congeners, and lower for some of the other congeners.
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Report the percent lipid content, so that the data user can calculate the concentration on a lipid basis if desired. The cleanup steps given in Section 13 of the method can be used to reduce or eliminate these interferences, permitting reliable determination of the CBs.
Observe the safety precautions in Section 5 and the recommendation in Section 5. The study design is detailed in an appendix to this epx. A new footnote 1 to Table 6 references the Ela interlaboratory study report, and the other footnote numbers are incremented. Possible causes may be loss by evaporation into the headspace of the sample container during shipment, with subsequent release to the atmosphere when the container is opened, or to biological or other degradation ep transit, although selective degradation of congeners at LOCs 1 and 2 only would be unusual.
Instrument internal standard 8. For drinking water or other samples containing low solids, smaller disks may be used. Dichlorobiphenyls, Total Report List.
Method A Interlaboratory Validation Study Report
In eps correspondence, the laboratory indicated that the lighter PCB congeners were lost during final transfer, and therefore, results were not submitted. Dilute the Labeled injection internal standard stock solution Section 7. Other quantitation references and procedures may be used provided that the results produced are as accurate as results produced by the quantitation references and procedures described in this Section.
The total number of analyses per laboratory could be upwards of 75 if calibration, QC, and a method detection limit MDL study are included.
Elution will be in the order of the di- ortho, mono-ortho, and non-ortho congeners. Concentration for Biosolids Figure is a plot of RSDs as a function of concentration for the congeners detected in biosolids. If an emulsion forms, the laboratory must employ mechanical techniques to complete the phase separation.
Prepare only the amount epx for each reference matrix with each sample batch. The reference compound for each compound is listed in Table 2. Recoveries for low molecular weight congeners are centered lower than for the other congeners and for recoveries of low molecular weight congeners in Method A.
Allow the acetone to spread evenly across the disk and soak for seconds. Weigh the receiver and boiling chips. The fraction discarded will contain residual 1668s and other potential interferents, if present.
Plots of absolute and relative precision as a function of concentration are addressed with precision for biosolids and tissue samples in Section 4. Alternatively, prepacked Florisil columns may be used.
Rpa properly adjusted, 1 to 2 drops of toluene will fall per second from the condenser rpa into ela receiver. This aliquot is used for determining the solids content of the sample, not for determination ofCBs.
The cleanup steps given in Section 13 can be used to reduce or eliminate these interferences and thereby permit reliable determination of the CBs at the levels shown in Table 2. The column specified in this Method is designed to handle a maximum of ig of a given CB, depending on the congener Reference These revised criteria are in Table of this report, and Table 6 of the successor method, B.
When handling samples suspected or known to contain high 1668aa of the CBs, an additional set of gloves can also be worn beneath the latex gloves. The tap water rinse is followed by another methanol rinse, then acetone, and then methylene chloride.
Common requests for analysis offish tissue include whole fish- skin on, whole fish-skin removed, edible fish fillets filleted in the field or by the laboratoryspecific organs, and other portions.
The estimated minimum levels EMLs in Table 2 are based on common laboratory contamination levels. It is also suggested that the laboratory perform personal hygiene monitoring of each analyst who uses this Method and that the results of this monitoring be made available to the analyst.
For example, the SPB-octyl column Section 6. The 1668w frequently encountered interferences are chlorinated dioxins and dibenzofurans, methoxy biphenyls, hydroxydiphenyl ethers, benzylphenyl ethers, brominated diphenyl ethers, polynuclear aromatics, polychlorinated naphthalenes, and pesticides.
Assistance in evaluating the health hazards of particular laboratory conditions may be obtained from certain consulting laboratories and from State Departments of Health or Labor, many of which have an industrial health service. Kansas City, KS Phone: To further reduce study costs, EPA plans to use excess sample volume collected from previous studies of biosolids and fish. Although PCBs are persistent, and thus do not require preservation, biosolids and tissue samples were shipped on ice to hinder decomposition of the tissues and gas formation in the biosolids.
The GC conditions may be optimized for compound separation and sensitivity.
In preparing Study samples, EPA’s objective was to ensure that the congeners present in each sample matrix would span the anticipated measurement range of 166a8 A, from the upper end of the calibration range down to “not detected.
Allow the receiver to dry. Because of the stability of PCBs, the samples will not require preservation. Prepare, extract, clean up, and concentrate the Method blank.
Standards should be prepared in volumes consistent with laboratory use to minimize the disposal of excess 1668 of expired standards.
Pull the acetone through the disk, releasing the vacuum when approx wpa mm thickness remains on the filter. This Method was prepared by Interface, Inc. If the ion a calibration point at 0. This eliminated concerns about how well spiked constituents would be incorporated into these matrices and whether spiked samples were representative of real-world samples.
The intent was to ensure that Study participants already possessed the facilities, equipment, and trained staff necessary to implement the method.
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